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WANG Mengling^ab LlU Minzhen^ab, WU Dan^c , WEl Jianling^d, GUO Changq^d, TAN Ning^c

(a.College of Pharmacy, b. Graduate School, c. College of Basie Medicine .d. College of Clinical Medicine, Guilin Medical University, Guilin 541 199, China)

Abstract Objective  To observe the effeet of Lanatoside C on the proliferation in hepatocellular carcinoma cellHepG2 and to explore its potential molecular mechanism. Methods HepG2 cells were treated with variousconcentrations of Lanatoside C , and the elfeet of Lanatoside C on the proliferation of HepG2 cells was evaluated bycell proliferation and clonal formation experiments. Flow cytometry was used to analyze the cell cycle. Western blotassay was used to test the protein expression levels of cell cycle related proteins p21, p27, CDK4, p-Akt and Akt.Results 'The proliferation of HepG2 cells was significantly inhibited by Lanatoside C in a dose-dependent manner.Compared with the control group, the clonogenesis rate of HepG2 cells was significantly decreased by Lanatoside C.Flow cytometry results showed that Lanatoside C blocked HepG2 cells in G0/G1 phase. Wester blot results showed that the protein expressions of p2l and p27 in HepG2 cells were significantly up-regulated by Lanatoside C , and theprotein expressions of p-Akt and Akt were significantly down-regulated. Conclusion Lanatoside C inhibites theproliferation and induces the cell cyele arrest in HepG2 cells, which the mechanism may be related to the inhibitionof Akt protein expression and phosphorylation induced by Lanatoside C, as well as the up-regulation of cell cyeleregulatory proteins p21 and p27 induced by Lanatoside C.Keywords:liver cancer:Lanatoside C:cell cycle : Akt

DOI:10.19296/j.cnki.1008-2409.2024-02-011

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