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LI Changqing¹, XIN Linfeng¹, QIN Qin¹, HUANG Huimei², NI Hui¹, LI Jiale¹, ZHANG Lin¹

(1.Affiliated Hospital of Guilin Medical University, Guilin 541001, China;

2.The People¡äs Hospital of Guigang City, Guigang 537100, China)

Abstract Objective To investigate the expression and role of the tenth exon of death receptor 3 ( DR3) in breast cancer cells. Methods Gene expression profiling interactive analysis ( GEPIA ) was used to analyze the expression and prognostic value of DR3 in breast cancer, and log-rank test was used to compare the survival differences. Construction of the plasmid vector splicing the tenth exon of DR3 was constructed and the tenth exon of DR3 in triple negative breast cancer cell line ( MDA-MB-231) was knocked down using CRISPR/Cas9 technology. Results Bioinformatics analysis showed that DR3 expression was low in breast cancer patients, and low expression predicted poor overall survival. In vitro experiments revealed that knockdown of the tenth exon of DR3 enhanced the expressions of DR3 transcript variants 2, 3 and 7, and this alteration resulted in enhanced proliferation of TNBC cell lines. Conclusion The low expression of DR3 in the patients with breast cancer is indicative of shorter overall survival, and the tenth exon of DR3 is closely related to breast cancer.

Keywords: the tenth exon of DR3; transcript variants; breast cancer; proliferation

DOI:10.19296/j.cnki.1008-2409.2024-03-006

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